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After 28 days mice were sacrificed and fresh striata were dissected for dopamine and 1-methyl-4-phenylpyridinium (MPP + ) analysis and midbrains were fixed in 4% paraformaldehyde for immunohistochemistry staining

Studies using the Fmr1 KO mouse model demonstrated that reactive astrocytes exacerbate oxidative stress by increasing ROS emission 97

Malabsorption due to digestive disorders, such as Crohns disease or celiac disease, can also lead to vitamin B12 deficiency

Prior to the behavioural procedures involving auditory startle responses (ASRs), rats were handled and acclimated (5 min) to the startle boxes and background noise (65 dB white noise)